FastQCFastQC Report
Thu 16 Mar 2017
H5M3MBCXY_l02n01_ah8921i-031517.35100000080fa9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5M3MBCXY_l02n01_ah8921i-031517.35100000080fa9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9213021
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG107344411.651379064478416TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1171000.044.6378631
TCGGAAG1173800.044.4438443
GCACACG1163900.044.3948411
AGCACAC1169500.044.31510
GAGCACA1171000.044.2908979
ATCGGAA1181150.044.2568322
ACACGTC1167450.044.21142213
CACACGT1168450.044.2024712
CGGAAGA1179550.044.1966634
AGAGCAC1176500.044.1546028
CACGTCT1167400.044.1535714
ACGTCTG1166850.044.08592215
CGTCTGA1171350.043.9033516
GAACTCC1171350.043.72303421
CGTCCCG1150850.043.69830335
GTCTGAA1177000.043.67348517
GTCCCGA1154800.043.6472536
TCCAGTC1170350.043.5926725
ACCCGTC1161250.043.56563632
TCACCCG1163650.043.56472830
Produced by FastQC (version 0.11.2)