FastQCFastQC Report
Thu 16 Mar 2017
H5M3MBCXY_l02n01_ah8921a-031517.35100000080f9c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5M3MBCXY_l02n01_ah8921a-031517.35100000080f9c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7809639
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG95040.1216957659630618TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG18150.027.6425611
ACACGTC20150.025.01053413
GATCGGA20600.024.9130121
CACACGT20900.024.4359712
ACGTCTG21050.023.94120215
CACGTCT21300.023.871314
CGTCTGA22500.022.99857516
AGCACAC23350.022.16108110
GAGCACA24250.021.3386129
CAGTCAC23550.020.54065327
GAACTCC24450.020.2453121
TCCAGTC24250.020.13354725
CCAGTCA24650.019.8981126
GTCACAT25450.019.18508329
CTCGTAT25650.019.12125244
AGAGCAC26600.019.115118
CACATGT26550.018.30547131
TCGGAAG28600.018.2557583
TCACATG26700.018.11836230
TCGTATG28200.018.0304545
Produced by FastQC (version 0.11.2)