FastQCFastQC Report
Thu 16 Mar 2017
H5M3MBCXY_l02n01_ah8920i-031517.35100000080f72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5M3MBCXY_l02n01_ah8920i-031517.35100000080f72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13600393
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG2962392.178164998614378TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA329100.042.445091
GCACACG325000.042.43625311
ACACGTC326450.042.2750213
CACGTCT328250.041.9609514
CACACGT329300.041.9023112
ACGTCTG328600.041.81401415
AGCACAC331350.041.74522410
GAGCACA333900.041.4668439
AGTTCCG327400.041.39917834
TATCTCG327700.041.38646341
TCGGAAG337750.041.3443833
GAACTCC332200.041.23371521
CCGTATC329050.041.17609438
TCCAGTC332600.041.0683725
GTTCCGT329950.041.052135
CAGTCAC332950.041.03180727
CGTCTGA334900.041.0277316
AGTCACA334250.040.87925328
CGTATCT330900.040.87094539
GTCACAG333500.040.8639929
Produced by FastQC (version 0.11.2)