FastQCFastQC Report
Thu 16 Mar 2017
H5M3MBCXY_l02n01_ah8920a-031517.35100000080f4c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5M3MBCXY_l02n01_ah8920a-031517.35100000080f4c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13371294
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG22350.023.85764711
CACACGT25500.020.8222212
ACACGTC25650.020.52502413
ACGTCTG26850.020.11069115
GATCGGA27850.019.7196161
CACGTCT28550.018.67658214
CGTCTGA29650.018.28748716
AGCACAC32050.017.26886710
GAGCACA33000.016.7035569
CACAGTC32000.016.5235131
GAACTCC32750.016.28202621
TCCAGTC33650.016.04689825
CAGTCAC33600.015.60204627
CTCGTAT35450.014.85096744
CCAGTCA35000.014.84939826
AGAGCAC38400.014.823348
TCGGAAG38650.014.7898193
AGTCACA35800.014.45493828
GTCACAG36750.014.38781829
ATCTCGT38300.013.86335742
Produced by FastQC (version 0.11.2)