FastQCFastQC Report
Thu 16 Mar 2017
H5M3MBCXY_l02n01_ah8868b-031517.35100000080f3f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5M3MBCXY_l02n01_ah8868b-031517.35100000080f3f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5004174
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG554181.1074355128338864TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG63500.041.45455615
GCACACG63950.041.268411
GATCGGA64750.041.0537221
ACACGTC64500.040.9513813
CACGTCT64900.040.59457414
CACGTGG64350.040.38495331
AGTCACG65000.040.32605428
CGTCTGA65500.040.22352216
TATCTCG64850.040.00179341
GGCCTTA65050.039.9841736
CGTGGCC64850.039.90010533
CACACGT66200.039.89975712
ACGTGGC65850.039.43085532
GAACTCC66550.039.28540821
CAGTCAC66900.039.1799927
CCAGTCA66900.039.11272426
AGCACAC68150.038.9891910
GTCACGT67000.038.98913629
TCCAGTC67300.038.9136925
GAGCACA68450.038.686839
Produced by FastQC (version 0.11.2)