FastQCFastQC Report
Thu 16 Mar 2017
H5M3MBCXY_l02n01_ah8868a-031517.35100000080f08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5M3MBCXY_l02n01_ah8868a-031517.35100000080f08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2976437
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC162090.5445772915737844TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGC19650.038.3592830
GATCGGA20300.038.0311551
CACGGCT19950.037.8952331
AGTCACG20100.037.49922628
GCACACG20500.037.42417511
GTCACGG20150.037.1841129
ACGGCTA20850.036.58321432
CACACGT20900.036.49262612
CACGTCT20900.036.49262614
ACGTCTG20900.036.38620415
ACACGTC20950.036.2981413
CGGCTAC20900.036.06506333
GGCTACA21200.035.7675834
CATCTCG21150.035.53058639
GTATGCC21500.035.2649445
CGTATGC21800.034.9860544
AGCACAC21950.034.84946410
CAGTCAC21800.034.4711927
GCTACAT22050.034.3893635
CGTCTGA22400.033.94962316
Produced by FastQC (version 0.11.2)