FastQCFastQC Report
Thu 16 Mar 2017
H5M3MBCXY_l02n01_ah8189b-031517.35100000080eed.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5M3MBCXY_l02n01_ah8189b-031517.35100000080eed.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3772037
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC132240.35057980608355643TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG17550.034.61377311
ACGTCTG18550.032.74823815
ACACGTC18700.032.60543413
GTATGCC18600.032.17592245
CGTCTGA19150.031.72260516
GATCGGA19450.031.7090551
CACACGT19300.031.7083712
TATCTCG19050.031.41627739
CACGTCT19350.031.39388814
CGTATGC18850.031.39111344
CACTAGC19100.030.98187831
CTCGTAT19600.030.07513242
TCGGAAG20650.029.7510813
TCACTAG20100.029.66437130
AGCACAC20550.029.56066710
GAGCACA20900.029.2809359
GAACTCC20600.029.05281621
TCCAGTC20800.028.88086725
ACTAGCT20750.028.84355732
CCAGTCA20750.028.84203126
Produced by FastQC (version 0.11.2)