FastQCFastQC Report
Thu 16 Mar 2017
H5M3MBCXY_l02n01_ah8189a-031517.35100000080ed0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5M3MBCXY_l02n01_ah8189a-031517.35100000080ed0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5267270
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC65120.12363140678188131TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG12150.027.0360928
GCACACG12500.026.09858911
ACGTCTG13350.024.94294715
GATCGGA13150.024.8186761
CGATCAG13150.024.6386233
GTCACGA13250.024.62224829
ACACGTC13950.023.70839913
GATCTCG13800.023.47743839
CACGTCT14400.023.1237514
CACACGT14450.022.88803712
CGTCTGA14900.022.49942816
CACGATC15050.021.97639331
CAGTCAC15500.021.19280827
AGCACAC16050.020.74654210
CGTATGC16000.020.67075744
GAGCACA16100.020.542379
TCCAGTC15900.020.5181525
AGAGCAC15900.020.517768
GTATGCC16350.019.9530545
ATCTCGT16200.019.86041640
Produced by FastQC (version 0.11.2)