FastQCFastQC Report
Thu 16 Mar 2017
H5M3MBCXY_l02n01_ah8187b-031517.35100000080eb6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5M3MBCXY_l02n01_ah8187b-031517.35100000080eb6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4435369
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC113050.2548829646417243TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG16900.033.41549311
GATCGGA17750.032.209011
ACACGTC17500.032.14125413
ACGTCTG18250.030.57416515
AGCACAC18600.030.24042510
CACGTCT18500.030.16065814
CACACGT18800.029.9187212
CGTCTGA18850.029.48228816
CCAGTCA19500.028.72933626
GAACTCC19600.028.4689321
GAGCACA20000.028.3485879
GTATGCC19500.028.15244145
CGTATGC19700.027.75242444
TCCAGTC20150.027.46761325
CAGTCAC20350.027.41878127
ATCTCGT20150.027.35626440
TCGGAAG21300.027.0473063
CTCGTAT20250.026.88754742
AGAGCAC21500.026.4754248
AGTCACC21000.026.35642428
Produced by FastQC (version 0.11.2)