FastQCFastQC Report
Thu 16 Mar 2017
H5M3MBCXY_l02n01_ah8187a-031517.35100000080e9d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5M3MBCXY_l02n01_ah8187a-031517.35100000080e9d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5280779
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC165910.31417713182089235TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG25400.035.2540511
ACACGTC26400.034.1743413
GATCGGA26800.034.1004941
ACGTCTG27000.033.66489415
CGTCTGA27150.033.3963516
CACACGT27050.033.18679412
TCCAGTC28050.032.16438725
CACGTCT28200.031.9129114
AGCACAC28300.031.64144510
GAGCACA28750.031.3809599
GTATGCC27900.031.36901945
CATCTCG27750.031.05330339
GAACTCC29750.030.70513721
CAGTCAC29100.030.6945527
CCAGTCA29500.030.50715826
AGTCACC29400.030.30510128
CGTATGC28850.030.18010344
AGAGCAC30250.029.9736298
TCGGAAG30950.029.4491883
CTCGTAT30950.028.20503442
Produced by FastQC (version 0.11.2)