FastQCFastQC Report
Thu 16 Mar 2017
H5M3MBCXY_l02n01_ah8038i-031517.35100000080e73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5M3MBCXY_l02n01_ah8038i-031517.35100000080e73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10694536
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC2816852.6339151132877574TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG305200.043.48628211
GATCGGA307950.043.4608351
ACACGTC307100.043.2023813
CACGTCT307300.043.115714
CACACGT308500.043.05008312
ACGTCTG307450.043.0300115
AGCACAC310500.042.84544810
CGTCTGA310900.042.5527116
GTATGCC307200.042.48523745
GAGCACA314250.042.3914459
TCGGAAG316000.042.2753373
CAGTCAC310000.042.24744427
AGAGCAC316400.042.153168
ACTGACC310600.042.10220732
TCCAGTC312300.042.0155525
GAACTCC315550.041.68421
AGTCACT314750.041.67440428
CCAGTCA314800.041.6675926
CGTATGC313200.041.6641644
GTCTGAA318450.041.50145317
Produced by FastQC (version 0.11.2)