FastQCFastQC Report
Thu 16 Mar 2017
H5M3MBCXY_l02n01_ah8038a-031517.35100000080e4d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5M3MBCXY_l02n01_ah8038a-031517.35100000080e4d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15370402
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG16150.011.84142111
TCCGATC20400.011.0287748
CACACGT20600.010.3755812
CCGATCT22400.010.0440629
ACACGTC20000.09.89939513
ACGTCTG21750.09.82708315
ACGCTCT23450.09.5023721
GTATGCC25350.08.34268645
CACGTCT23800.08.3188214
TATCTCG24000.07.96844539
CCGATGT25450.07.691607533
AGCACAC26700.07.66809610
CGCTCTT28850.07.6444532
CGTCTGA29400.07.499712516
GAACTCC29250.07.38454821
GATCGGA26150.07.31622271
CAGTCAC27900.07.25776327
TCCAGTC30900.06.771629325
CGATGTA31000.06.67757634
CGTATGC30450.06.57595344