FastQCFastQC Report
Thu 16 Mar 2017
H5M3MBCXY_l02n01_ah6978b-031517.35100000080e30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5M3MBCXY_l02n01_ah6978b-031517.35100000080e30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6353932
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC3124924.918088515898502TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA342400.044.2709541
GCACACG343650.043.79205311
ACACGTC345400.043.49167613
CACACGT346600.043.40600612
CACGTCT346250.043.3654114
GTCACGC343750.043.3638529
GAGCACA348550.043.3636059
AGCACAC347800.043.33421310
ACGTCTG347150.043.2737915
AGTCACG346450.043.20638328
CGTCTGA347750.043.18618816
AGAGCAC351050.043.0804258
TCACGCC345500.043.04000530
TCGGAAG352400.043.0253753
CACGCCA345700.042.9630431
TCCAGTC348850.042.84362825
GTATGCC348250.042.77396445
CAGTCAC349850.042.7600927
TATCTCG346650.042.7066439
ATCGGAA355750.042.6645052
Produced by FastQC (version 0.11.2)