FastQCFastQC Report
Thu 16 Mar 2017
H5M3MBCXY_l02n01_ah6978a-031517.35100000080e09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5M3MBCXY_l02n01_ah6978a-031517.35100000080e09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4682959
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC747381.59595674444299TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA87950.041.8178941
GCACACG86950.041.7378311
CACACGT87150.041.64204812
CACGTCT87300.041.33810414
CGTCTGA87450.041.3195416
ACACGTC87800.041.3081413
ACGTCTG87500.041.2187915
AGCACAC89000.040.95341510
GATCTCG87750.040.92139
GTCACAC87550.040.7840729
GTATGCC88650.040.75935445
GAGCACA89800.040.7138449
CACACAG88100.040.4021931
AGAGCAC91450.040.1022728
TCCAGTC90200.040.03399725
TCGGAAG92100.039.976923
GAACTCC90450.039.94907421
CCAGTCA90500.039.90128726
CACAGTG89500.039.77020333
ATCTCGT90600.039.75791540
Produced by FastQC (version 0.11.2)