FastQCFastQC Report
Thu 16 Mar 2017
H5M3MBCXY_l01n01_ah9042i-031517.35100000080fb5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5M3MBCXY_l01n01_ah9042i-031517.35100000080fb5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9283174
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG4049614.3623118558372385TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA455950.044.253771
GCACACG454200.043.89473711
ACACGTC455850.043.60540813
CACACGT458100.043.54559312
ACGTCTG456650.043.488915
CACGTCT457400.043.41266614
AGCACAC460750.043.3830410
TCGGAAG465150.043.3454863
GAGCACA461150.043.321029
CGTCTGA458450.043.2936116
AGAGCAC462950.043.215768
AGTCACG455250.043.08605228
ATCGGAA471200.042.879932
GTCACGT457600.042.82053429
CACGTGA457750.042.79313731
TCACGTG458650.042.7028830
TCCAGTC459600.042.58432425
GATCTCG454600.042.55846841
CGGAAGA473600.042.5388534
GAACTCC461850.042.52742421
Produced by FastQC (version 0.11.2)