FastQCFastQC Report
Thu 16 Mar 2017
H5M3MBCXY_l01n01_ah9042a-031517.35100000080f8f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5M3MBCXY_l01n01_ah9042a-031517.35100000080f8f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13258112
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG2547921.9217819249075583TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA300900.042.8677441
GCACACG295350.042.65160411
CACACGT299500.042.06060412
ACACGTC298150.042.03141813
ACGTCCG292200.041.95078332
AGCACAC302500.041.72529210
CGTCCGC293000.041.67497633
ACGTCTG301300.041.487315
GAGCACA305400.041.4616859
AGTCACG299000.041.38661628
CCGCACA294350.041.3454736
CACGTCT302700.041.3325814
CGTCTGA304200.041.09179716
TCGGAAG314150.041.070773
GTCCGCA297200.041.0109434
GTCACGT302100.040.9034329
AGAGCAC311200.040.8118448
CATCTCG301650.040.7097741
CACGTCC301250.040.63823731
TCACGTC305000.040.42598730
Produced by FastQC (version 0.11.2)