FastQCFastQC Report
Thu 16 Mar 2017
H5M3MBCXY_l01n01_ah8963k-031517.35100000080f65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5M3MBCXY_l01n01_ah8963k-031517.35100000080f65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8450940
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG105694212.506798060334118TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1167200.044.5949481
TCGGAAG1166400.044.559573
GCACACG1156300.044.47912211
CGGAAGA1168900.044.4353874
AGCACAC1160700.044.42487710
ATCGGAA1171950.044.421782
GAGCACA1161700.044.407949
CACACGT1161550.044.305212
ACACGTC1160650.044.29727613
CACGTCT1160150.044.226914
ACGTCTG1159600.044.1819115
AGAGCAC1170950.044.124398
CGTCTGA1164550.043.9651316
GAACTCC1159550.043.88993521
GTCTGAA1166800.043.8610717
TCTGAAC1167100.043.8208818
TCCAGTC1157350.043.7150725
TGAACTC1167800.043.71473320
TCGTATG1144100.043.6960245
ACTCCAG1160800.043.67236323
Produced by FastQC (version 0.11.2)