FastQCFastQC Report
Thu 16 Mar 2017
H5M3MBCXY_l01n01_ah8963j-031517.35100000080f59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5M3MBCXY_l01n01_ah8963j-031517.35100000080f59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9353182
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG95250.10183700049886765TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG18750.025.91917411
ACGTCTG21200.022.81681415
ACACGTC21950.022.24219513
CACACGT22450.021.547212
GATCGGA22600.021.512481
CGTCTGA23300.020.76036516
CACGTCT23400.020.57549914
AGCACAC25700.019.69781710
GAGCACA26250.018.6851189
CAGTCAC25600.018.45614827
TCCAGTC26300.018.05046725
CCAGTCA26650.017.64455826
GAACTCC27900.017.57980321
TCACATG26650.017.30704730
CTCGTAT27450.017.1300544
CACATGT27200.016.95718231
AGTCACA28050.016.68378328
AGAGCAC29300.016.5097128
GTCACAT29600.016.19027529
TCGGAAG30250.016.144733
Produced by FastQC (version 0.11.2)