FastQCFastQC Report
Thu 16 Mar 2017
H5M3MBCXY_l01n01_ah8919k-031517.35100000080f22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5M3MBCXY_l01n01_ah8919k-031517.35100000080f22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7469991
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG2177882.9155055206893823TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA246100.043.7811
GCACACG243900.043.7358811
CACACGT245650.043.4151512
ACGTCTG245400.043.3733815
ACACGTC246150.043.34234213
CACGTCT245900.043.3129214
AGCACAC249500.042.86245310
CGTCTGA248400.042.8404916
GAGCACA250400.042.735359
CCGTATC245600.042.45148538
AGTTCCG245550.042.43462434
AGAGCAC252550.042.3982628
TCGGAAG254050.042.3878483
CAGTCAC249100.042.3424727
GTTCCGT247250.042.2705535
TCCAGTC250450.042.18609625
TATCTCG246750.042.10774241
GTCACAG249200.042.0464429
ATCGGAA257050.041.990292
GAACTCC253100.041.9127421
Produced by FastQC (version 0.11.2)