FastQCFastQC Report
Thu 16 Mar 2017
H5M3MBCXY_l01n01_ah8919j-031517.35100000080f15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5M3MBCXY_l01n01_ah8919j-031517.35100000080f15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12226188
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG19150.022.32243511
CACACGT23000.018.97713512
ACACGTC23250.018.86930813
GATCGGA23350.018.2186581
ACGTCTG24700.017.85260815
CACGTCT24800.017.32703414
CGTCTGA26550.016.52390316
AGCACAC27100.015.94001310
CATCTCG24500.015.79543239
GTATGCC26750.015.64380445
GAGCACA29250.014.999119
CAGTCAC28200.014.44069227
CGTATGC29850.013.79303844
AGAGCAC32750.013.4648498
CCAGTCA31900.013.32998526
TCCAGTC31950.013.09786825
CTCGTAT30950.013.08479142
GAACTCC32350.013.07495821
AGTCACC32800.012.62156428
TCGGAAG37900.011.9953963
Produced by FastQC (version 0.11.2)