FastQCFastQC Report
Thu 16 Mar 2017
H5M3MBCXY_l01n01_ah8869b-031517.35100000080efa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5M3MBCXY_l01n01_ah8869b-031517.35100000080efa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3187779
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG330161.0357054237448706TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA39900.041.4020461
GCACACG39550.041.18645511
ACACGTC39700.040.8608313
CACGTCT39850.040.76347714
ACGTCTG40300.040.47579215
CGTCTGA40800.039.8143316
CACACGT41150.039.5303712
AGCACAC41650.039.10983310
AGAGCAC42150.038.8060268
CACAGTC41050.038.75094231
GAGCACA42200.038.653429
CAGTCAC41550.038.50062627
TCCAGTC41950.038.4553225
CCAGTCA42100.038.2114226
GAACTCC42550.038.07168621
TCGTATG42150.037.9525745
CTCGTAT41800.037.78592344
TCGGAAG43850.037.7215163
AGTCACA42600.037.71011428
GTCACAG42350.037.66708429
Produced by FastQC (version 0.11.2)