FastQCFastQC Report
Thu 16 Mar 2017
H5M3MBCXY_l01n01_ah8869a-031517.35100000080ec3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5M3MBCXY_l01n01_ah8869a-031517.35100000080ec3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4176793
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC1597083.8236991873908996TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG172550.043.83851211
GATCGGA173950.043.695921
ACACGTC172900.043.6175513
CACACGT173750.043.50984212
AGCACAC174800.043.29997310
ACGTCTG173500.043.27168315
CACGTCT174200.043.26569714
GTATGCC171150.043.2485245
CGTCTGA173950.043.13387716
TCGGAAG176450.043.0844153
GAGCACA176150.043.044769
AGAGCAC176650.042.973878
CAGTCAC174500.042.74159627
TCCAGTC175250.042.64855225
CCAGTCA175050.042.60730726
GAACTCC176000.042.5046521
CGTATGC175200.042.30013344
GTCACCT175800.042.2601729
GTCTGAA177750.042.1737717
CTCGTAT174250.042.15631542
Produced by FastQC (version 0.11.2)