FastQCFastQC Report
Thu 16 Mar 2017
H5M3MBCXY_l01n01_ah8867b-031517.35100000080eaa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5M3MBCXY_l01n01_ah8867b-031517.35100000080eaa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3464837
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATG143780.414969015858466TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA19000.037.1969381
ACACGTC19200.036.5591813
CACACGT19150.036.42072312
CACGTCT19450.036.08874514
GCACACG19400.036.06735611
ACGTCTG19550.035.67399615
CGTCTGA20100.034.69784516
TATCTCG20100.034.25309441
GAGCACA21050.033.4539879
AGAGCAC21500.032.649148
AGCACAC21700.032.45191210
CAGTCAC21350.032.3511627
CCAGTCA21650.032.31854626
TCCAGTC21700.032.03672825
ACTCCAG22050.031.93633823
TCGTATG21700.031.8298345
CTCGTAT21600.031.56054544
TCGGAAG22550.031.2381323
GTCACAT22400.030.93737229
GAACTCC22700.030.92274721
Produced by FastQC (version 0.11.2)