FastQCFastQC Report
Thu 16 Mar 2017
H5M3MBCXY_l01n01_ah8867a-031517.35100000080e80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5M3MBCXY_l01n01_ah8867a-031517.35100000080e80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3780589
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATG86190.2279803490937523TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG12150.032.40522811
ACACGTC12400.031.56962213
GATCGGA13250.029.8958531
CACACGT13550.028.89104312
ACGTCTG13850.028.42693715
CGTCTGA14050.028.02228416
GTCACAC13650.027.85637529
CACGTCT14350.027.27967314
AGCACAC14950.026.78749510
TATCTCG14400.026.4055241
CAGTCAC14600.026.04242327
CACACTG14600.025.58317831
GAGCACA15600.025.2386869
AGAGCAC15800.025.0616048
TCGGAAG16400.024.839193
CCAGTCA15350.024.7699926
CTCGTAT15650.024.15236944
TCGTATG16100.024.03628545
GAACTCC16500.023.99806821
AGTCACA16150.023.68324328
Produced by FastQC (version 0.11.2)