FastQCFastQC Report
Thu 16 Mar 2017
H5M3MBCXY_l01n01_ah7797k-031517.35100000080e66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5M3MBCXY_l01n01_ah7797k-031517.35100000080e66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11089542
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC2764012.4924473887199308TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG310600.043.4683511
GATCGGA315350.043.201151
ACACGTC313250.043.04238513
CACACGT313850.043.00388712
GTCACGC309150.042.96979529
CACGTCT314500.042.79977414
ACGTCTG315100.042.6825815
AGCACAC316750.042.67408810
TCACGCC310250.042.65789830
AGTCACG313000.042.55531328
CGTCTGA316300.042.47085616
GAGCACA321450.042.211129
CACGCCA314200.042.11578431
GTATGCC314250.042.08381345
TCGGAAG324350.042.053753
TATCTCG314050.041.84667239
CAGTCAC319400.041.79286227
AGAGCAC325750.041.7022638
TCCAGTC320750.041.60293625
GAACTCC321400.041.532821
Produced by FastQC (version 0.11.2)