FastQCFastQC Report
Thu 16 Mar 2017
H5M3MBCXY_l01n01_ah7797j-031517.35100000080e5a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5M3MBCXY_l01n01_ah7797j-031517.35100000080e5a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71818680
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC1954640.272163175374429TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG276650.034.68590511
ACGTCTG299650.031.88710215
ACACGTC301150.031.86290713
GTCACAC293450.031.85744529
CACACGT302000.031.82650812
GATCTCG296800.031.49000239
GATCGGA312150.031.0048791
CACGTCT311350.030.86226814
CGTCTGA316850.030.31944516
CACACAG309000.030.29147131
AGCACAC319450.030.2499710
GTATGCC314000.030.05085245
GAGCACA332950.029.1383119
CAGTCAC331000.028.534827
CGTATGC331650.028.50585244
GAACTCC343700.027.86004821
AGTCACA340650.027.74683628
TCCAGTC343150.027.64251925
CTCGTAT343050.027.3881842
CCAGTCA349650.027.07073426
Produced by FastQC (version 0.11.2)