FastQCFastQC Report
Thu 16 Mar 2017
H5M3MBCXY_l01n01_ah6408k-031517.35100000080e23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5M3MBCXY_l01n01_ah6408k-031517.35100000080e23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10246247
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC3921103.8268646071093158TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG428600.044.16838511
ACACGTC429700.043.9809413
CACACGT430500.043.9577712
ACGTCTG429200.043.90594515
AGCACAC432250.043.83185610
CACGTCT430600.043.78931414
GAGCACA433800.043.7115449
TCGGAAG437400.043.483033
GTATGCC426650.043.46808245
GAACTCC433250.043.32064421
GATCGGA439800.043.280251
CCAGTCA432950.043.2365526
CAGTCAC432850.043.22054727
TCCAGTC433500.043.1816925
CGTCTGA436650.043.14137616
AGTCACT434100.043.01400428
GTCTGAA438700.042.98593517
CGGAAGA442950.042.9331254
ACTCCAG437400.042.894423
TCTGAAC439050.042.8799318
Produced by FastQC (version 0.11.2)