FastQCFastQC Report
Thu 16 Mar 2017
H5M3MBCXY_l01n01_ah6408j-031517.35100000080e16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5M3MBCXY_l01n01_ah6408j-031517.35100000080e16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11680726
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC139730.11962441375647369TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG25250.028.87053111
CACACGT28600.025.56751312
ACACGTC30000.024.37300713
ACGTCTG30400.024.05210315
CACGTCT32700.022.7043714
AGCACAC32700.022.29299710
GATCGGA33400.022.1701851
GTATGCC32550.021.84190645
CGTCTGA34850.021.23911516
TATCTCG33050.021.1718239
CAGTCAC33800.021.16754227
CCGATGT33500.020.95575133
GAACTCC35900.020.4305221
GAGCACA37400.019.9125829
CTCGTAT36200.019.70184942
AGTCACC36850.019.41604628
TCCAGTC37150.019.31932325
GTCACCG37300.019.18180529
CCAGTCA37250.019.14665626
CGTATGC37200.019.11166844
Produced by FastQC (version 0.11.2)