FastQCFastQC Report
Wed 8 Mar 2017
H5LWJBCXY_l02n01_robot_protoplast_2.3510000007eebb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LWJBCXY_l02n01_robot_protoplast_2.3510000007eebb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9032919
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG2314672.562482847460494No Hit
CTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTAC2180502.4139483593288062No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC1830392.02635493576329TruSeq Adapter, Index 2 (100% over 51bp)
CCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACA1639911.8154817949767956No Hit
GTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAGGAGCATAG1633171.8080201981220023No Hit
CTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACAT1630131.8046547301044102No Hit
CTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACA1361271.5070100816801302No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGC1235841.3681513140990194No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA1113201.2323812490735275No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAG1085111.2012838817662375No Hit
GTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACT1036951.1479677831717523No Hit
GGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTT959701.0624472554220845No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGAT958001.0605652502806677No Hit
GTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC941071.0418226931958539No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAG845760.9363086284732542No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG804570.8907087509585773No Hit
GTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTG755690.8365955678336096No Hit
GTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTA753440.8341046786758523No Hit
ATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGT693220.7674374141957876No Hit
GGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTT663110.7341037819557553No Hit
CTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAG661290.7320889293925917No Hit
GCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTA649060.7185495629928709No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG619830.6861901451789837No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGG617050.6831125132418435No Hit
AACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAA614310.6800791637786191No Hit
GTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA612140.6776768395686931No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACT594510.6581593391903547No Hit
ATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGT582990.6454059867026374No Hit
GTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGA569650.6306377816517562No Hit
TGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTA564950.6254345909666632No Hit
GATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAGGAGCATAGGCTA548640.6073784122275424No Hit
CTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAAC533350.5904514365732716No Hit
ATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGT483780.5355743807732584No Hit
GTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTG482200.5338252230535888No Hit
GGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGA476200.5271828519662359No Hit
GTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGG470780.5211825767506606No Hit
CAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATC464320.5140309572132773No Hit
AGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGAT461380.5107761953804745No Hit
GCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAA458770.5078867639574759No Hit
TTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACA446870.4947127279675595No Hit
CTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGG445340.4930189233402845No Hit
CACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAA444740.4923546862315493No Hit
GTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGT429000.474929532745727No Hit
GTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTC428980.47490739150876915No Hit
CTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTAC422340.4675565008387654No Hit
CAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGT394780.4370458763108581No Hit
CTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATT385490.4267612717439402No Hit
CCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAG370690.4103767563951365No Hit
GGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAGGAG358620.3970145198910784No Hit
AGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGT350190.3876819885133477No Hit
CATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACC340520.3769767004442307No Hit
GTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGAT337100.3731905489244396No Hit
AGACACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTAACC332370.3679541463839098No Hit
TAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTG331160.36661460154796033No Hit
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATC322860.3574259882104555No Hit
TGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG317840.35186853773403703No Hit
TAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGA296630.3283877559402448No Hit
GTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGG286160.3167968183928141No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGTAACCTA284750.3152358611872862No Hit
CTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAG282570.31282246635888133No Hit
CCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTA267800.29647116286551445No Hit
CTAGAGTACAACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACTGG264750.29309462422944343No Hit
TAGACACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTAAC257060.2845813186191529No Hit
GCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACAGTC256270.2837067397593181No Hit
GTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGCTATCA249350.27604587177190454No Hit
AGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCA240100.26580554967890224No Hit
TAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTG234240.2593181672502543No Hit
AAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGA232650.2575579389121058No Hit
TCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT229030.2535503750227363No Hit
GTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAG220950.24460531529176782No Hit
GTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGT214960.2379740148228939No Hit
CTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACAGTCC210890.2334682731019729No Hit
TACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATA203690.2254974277971495No Hit
GAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAG200130.2215562876186535No Hit
TACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCT194430.21524603508566834No Hit
CTAGGTTACTCTGTAGGGTGTCTATGTACTAGGAGCATAGGCTACTAGTGG192180.212755145927911No Hit
CTGTTACTACCCTACACTCATACTTGGATCACACTATGTACTAGGAGCATA189580.20987678512339147No Hit
AGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGA189170.20942288976575568No Hit
GTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTA180550.19988001663692545No Hit
GTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCTCCTAGTACATAG180520.19984680478148867No Hit
GTTTGGTACTATCTGCTATGTATCCTGTCCTACTATCTAACCTCTGTAGGA180170.19945933313472644No Hit
ACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAAT179600.19882830788142794No Hit
TGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTA176700.19561782852254073No Hit
CAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACC176350.19523035687577847No Hit
CCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTA170290.18852156207755214No Hit
AGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCT168470.18650670951438844No Hit
GACTGTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAGGAGC168370.1863960033295992No Hit
TATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACA168220.1862299440524154No Hit
CTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTGT163540.1810488946042802No Hit
CTGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA162470.17986433842703559No Hit
TACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGG159460.17653208226488026No Hit
GCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTA159410.17647672917248566No Hit
CACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGA158770.1757682095898347No Hit
CCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGT157350.17419618176582785No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGG157040.17385299259298131No Hit
TAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAGG154350.17087499622215144No Hit
CTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACATAG152810.16917012097639755No Hit
CACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGA148540.16444296688589813No Hit
GTTACTCTGTAGGGTGTCTATGTACTAGGAGCATAGGCTACTAGTGGCTGT146550.16223991380859276No Hit
CTGTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAGGAGCAT144390.15984866021714575No Hit
CTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTAACCTCTGTAG142420.15766774837679823No Hit
CCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCT140670.155730390142987No Hit
CCTAGATTGATCAAACAGTCCTACTATCTAACCTCTGTAGGACTATCAGGT139620.15456797520270027No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGT136970.15163426130578608No Hit
CATAGACACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTA134850.14928729018825476No Hit
CTGCTATGTATCCTGTCCTACTATCTAACCTCTGTAGGACTATCAGGTGTC134460.14885553606757682No Hit
TTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCC134430.14882232421214006No Hit
CTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTT129900.14380733404118867No Hit
ACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTA129750.14364127476400484No Hit
AGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTA128800.14258956600850733No Hit
ACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGG126380.13991047633660836No Hit
AGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTA126180.13968906396702993No Hit
ATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAGGAGCATAGGCTAC124450.13777384697017653No Hit
TCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACAC123920.1371871041907937No Hit
CTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATCAATCTAGGTT120730.13365557689601779No Hit
TGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCT120050.13290277483945112No Hit
AACAACTAGAGTACAACAGCCACTAGTAGCCTATGCTCCTAGTACATAGAC119830.13265922123291485No Hit
ATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCTCCTAG119590.13239352638942073No Hit
CTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGT117110.12964801300664824No Hit
GTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGTAACCTAGAT116860.12937124754467522No Hit
AGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAGGA115840.1282420444598252No Hit
CCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTAC115800.12819776198590954No Hit
CTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAG114240.1264707455031978No Hit
TAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCA113060.1251644125226851No Hit
TGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACATAGC108030.11959589142778763No Hit
CAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCTC107750.11928591411037782No Hit
GTCCACTCGGTGAGCTTCTCGAAGTTTATGATGCTCTCCAGCTGCTCCAGC107100.11856632390924793No Hit
AGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCT105250.11651825949064748No Hit
CTAACCTCTGTAGGACTATCAGGACTGTTTGATCAATCTAGGTTACTCTGT103500.11458090125683623No Hit
GTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGTAACCTAGATTGA102920.11393880538505881No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGT101870.11277639044477207No Hit
TACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTAACCTCTGTAGG101500.11236677756105197No Hit
GATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTAC99390.11003087706199957No Hit
AGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTATGT97950.10843670800103487No Hit
AGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTG96010.10628900801612413No Hit
CTGGTCCACTCGGTGAGCTTCTCGAAGTTTATGATGCTCTCCAGCTGCTCC95700.10594581884327758No Hit
ACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGAC94350.10445128534862318No Hit
TGTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAGGAGCATA93830.10387561318771928No Hit
AGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAG93080.10304531680180017No Hit
CTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGAT92810.10274641010286928No Hit
GGACAGGATACATAGCAGATAGTACCAAACCCAGTGTCTATGTACTAGGAG91960.10180540753216098No Hit
GTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAAACAGTCCTACTATC91910.10175005443976637No Hit
TAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCTCCTAGTACATAGA91780.10160613639954039No Hit
GTTAGATAGTAGGACTGTTTGATCAAACAGTCCTACTATCTAACCTCTGTA90390.10006732043097032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATACG302.1676024E-644.998674
GTATGCC195250.044.1464345
TATCTCG195050.044.0527939
GTATCTC195050.044.0527938
CTCGTAT195350.044.03120842
TCTCGTA195400.044.0199441
ATCTCGT195450.043.98565340
CGTATGC196000.043.94282544
CCAGTCA199250.043.9259326
CCGATGT196550.043.91143833
ACCGATG196100.043.90894332
CAGTCAC198450.043.8989327
CGATGTA196350.043.88741334
TCCAGTC212300.043.87529825
TCGTATG196150.043.87456543
AGTCACC198300.043.8413728
AACTCCA213800.043.84108422
GATGTAT196550.043.83130635
CTGAACT213900.043.83110419
GAACTCC213950.043.8103521
Produced by FastQC (version 0.11.2)