FastQCFastQC Report
Wed 8 Mar 2017
H5LWJBCXY_l02n01_robot_protoplast_1.3510000007ee92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LWJBCXY_l02n01_robot_protoplast_1.3510000007ee92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8678115
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG3418543.939265612405459No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC1694221.9522903303309531TruSeq Adapter, Index 1 (100% over 51bp)
CTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTAC1631391.8798898147812053No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA1302321.5006945632778548No Hit
CCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACA1279741.4746750878502992No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAG1178231.357702680824119No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGC1048721.2084652024085876No Hit
GGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTT1045991.2053193579481258No Hit
CTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACAT1040121.198555216196144No Hit
GTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACT994631.14613599842823No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG942951.086583895235313No Hit
GTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAGGAGCATAG924901.0657844474289635No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGAT892731.0287141850505552No Hit
CTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACA857240.9878182070645527No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG854720.9849143506395109No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGG842250.9705448706314677No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAG811180.9347421646290698No Hit
GTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC733960.8457597070331517No Hit
GTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTG649620.7485727027125131No Hit
ATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGT610930.7039892879962988No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACT597680.6887209952852665No Hit
GGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTT584720.6737868765279096No Hit
GTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTG584100.673072435661431No Hit
ATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGT571050.6580346077460371No Hit
AACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAA566180.6524227899722462No Hit
GTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA559350.6445524172012009No Hit
TGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTA512610.5906927944605481No Hit
GCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTA502810.5794000194742752No Hit
GTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTA482400.5558810870793945No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGTAACCTA480030.5531500792510815No Hit
GTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGA468110.5394143774310435No Hit
CTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAG462390.5328230842757903No Hit
AGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGT448230.5165061767446041No Hit
CCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTA440500.5075987123931868No Hit
GTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGCTATCA438890.5057434707882991No Hit
CTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAG432030.49783852829790803No Hit
CAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATC419080.48291593278033307No Hit
AGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGAT407230.46926089363876833No Hit
CTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAAC402690.46402934277778063No Hit
TAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTG377590.43510601092518364No Hit
CACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAA371830.42846862481080283No Hit
TTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACA352510.4062057255521504No Hit
GCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAA344330.3967797154105471No Hit
GGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGA341350.3933457899555376No Hit
GTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGT337220.38858669192560824No Hit
GTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGG322970.37216607523638484No Hit
CAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGT322840.37201627311922003No Hit
GTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTC315030.3630166228495474No Hit
CTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATT314740.362682448895872No Hit
TGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG306930.3536827986261994No Hit
GATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAGGAGCATAGGCTA301900.347886609015898No Hit
CATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACC294010.33879477282797016No Hit
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATC291490.3358909164029285No Hit
TCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT291070.3354069403320883No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGT290660.3349344875010299No Hit
CCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAG287370.33114334161278114No Hit
ATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGT287110.3308437373784514No Hit
TAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGA274120.3158750489017488No Hit
GTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGAT273500.3151606080352703No Hit
CTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGG264780.3051123429454438No Hit
GGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAGGAG262570.30256570695364143No Hit
AAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGA249590.28760854171672073No Hit
GAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAG244560.2818123521064194No Hit
GTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGG235510.2713838201037898No Hit
CTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTAC222380.25625380627014044No Hit
AGACACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTAACC221160.25484797101674733No Hit
CTGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA219150.25253179982058316No Hit
AGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCA216950.2499966870685627No Hit
TGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTA210770.24287532488334163No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGG209450.2413542572321293No Hit
TACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGG199130.229462273777197No Hit
GTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGT197920.22806796176358576No Hit
CAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGC189230.21805426639310496No Hit
TAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTG173540.19997430317528633No Hit
GCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACAGTC173110.19947880386466413No Hit
TAGACACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTAAC171230.19731243478566485No Hit
GTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTA169220.19499626358950073No Hit
GTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAG167770.1933253938211236No Hit
GTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGTAACCTAGAT167450.19295665014810245No Hit
TACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCT163080.18792099436340726No Hit
CAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACC162430.187171983777583No Hit
AGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGA160810.1853052189329134No Hit
ACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGG156070.17984320327628753No Hit
ACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAAT153850.17728504404470327No Hit
CTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTGT152600.17584463907196435No Hit
TACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATA149840.17266422489215688No Hit
CCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGT148940.17162713331178486No Hit
GTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGTAACCTAGATTGA146170.1684351958921955No Hit
GTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAAACAGTCCTACTATC145380.1675248599494245No Hit
TATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACA144900.16697174443989277No Hit
AGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCT135910.15661235187595463No Hit
GTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGACTATCA134360.1548262497097584No Hit
CTAGGTTACTCTGTAGGGTGTCTATGTACTAGGAGCATAGGCTACTAGTGG133940.15434227363891811No Hit
CTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGT132760.15298253134465262No Hit
GCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTA127850.14732462061173424No Hit
ACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTA127660.1471056790558779No Hit
CTGTTACTACCCTACACTCATACTTGGATCACACTATGTACTAGGAGCATA127480.14689826073980353No Hit
CCTAGATTGATCAAACAGTCCTACTATCTAACCTCTGTAGGACTATCAGGT127100.1464603776280909No Hit
CTCTAATGCTGGACACCTGATAGTCCTACAGAGTAACCTAGATTGATCAAA126870.14619534311310695No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGT126340.14558461140466564No Hit
GTTAGATAGTAGGACTGTTTGATCAAACAGTCCTACTATCTAACCTCTGTA126090.14529653041011786No Hit
CACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGA125960.14514672829295303No Hit
CCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTA124810.14382155571803323No Hit
GGACTGTTTGATCAATCTAGGTTACTCTGTAGGGCTATCAGGTGTCCAGCA124750.14375241627934177No Hit
CTAGAGTACAACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACTGG124480.1434412888052302No Hit
CTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTT123560.14238115074529434No Hit
CTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACAGTCC121480.13998431687065682No Hit
CAGAGGTTAGATAGTAGGACTGTTTGATCAAACAGTCCTACTATCTAACCT117650.13557091603418484No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGT117490.13538654419767426No Hit
TAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAGG114520.13196414198244666No Hit
GACTGTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAGGAGC112160.12924465739391564No Hit
CTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACATAG112090.12916399471544224No Hit
TAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCA111860.1288989602004583No Hit
CTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATCAATCTAGGTT110740.12760835734488424No Hit
CACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGA110280.12707828831491633No Hit
CTGCTATGTATCCTGTCCTACTATCTAACCTCTGTAGGACTATCAGGTGTC109320.12597205729585284No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGTAAC106780.12304515439124741No Hit
GTTTGGTACTATCTGCTATGTATCCTGTCCTACTATCTAACCTCTGTAGGA106350.12254965508062524No Hit
CTAACCTCTGTAGGACTATCAGGACTGTTTGATCAATCTAGGTTACTCTGT105100.12110925010788633No Hit
GTTTGATCAATCTAGGTTACTCTGTAGGGCTATCAGGTGTCCAGCATTAGA104990.1209824944702853No Hit
GATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTAC104900.1208787853122481No Hit
AGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAGGA104670.12061375079726415No Hit
TTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCC103130.11883917187084983No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGCCCTACAGAG102580.11820539368284473No Hit
CTGTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAGGAGCAT101980.11751399929593005No Hit
CTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAG101150.11655757039403143No Hit
CCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCT100970.11635015207795703No Hit
AGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGCTATCAGGT99810.11501345626325533No Hit
TGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCT99460.11461014287088844No Hit
AGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTA96930.11169476320606492No Hit
TCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACAC95890.11049634626874615No Hit
CCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTAC94940.10940163848946459No Hit
GTGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA94840.10928640609164549No Hit
CATAGACACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTA94820.10926335961208165No Hit
CTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTAACCTCTGTAG93220.10741964124697588No Hit
GTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGCTATCAGGTGTCCA93130.10731593208893866No Hit
AGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAG92140.10617513135052947No Hit
GTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGTA90700.10451578482193426No Hit
CTACAGAGGTTAGATAGTAGGACTGTTTGATCAAACAGTCCTACTATCTAA89570.1032136587265783No Hit
GTTACTCTGTAGGGTGTCTATGTACTAGGAGCATAGGCTACTAGTGGCTGT87920.10131232416256296No Hit
AGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTA87550.10088596429063224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT518950.044.0092121
GTATGCC176750.043.892145
CTCGTAT176500.043.8897742
ATCTCGT176250.043.888240
ACGATCT176100.043.8489237
TCTCGTA176550.043.83910841
TCACGAT176600.043.8012235
CACGATC176500.043.80053736
TCGTATG177200.043.75448243
GATCTCG176650.043.7506139
CGTATGC177600.043.66885444
CATCACG177500.043.65517833
GTCACAT177850.043.5690229
ATCACGA177750.043.53049534
CAGTCAC179600.043.45767227
CACATCA178400.043.42233331
CCAGTCA181650.043.351226
TCACATC178800.043.28719330
ACATCAC179450.043.1933432
TCCAGTC192450.043.17475525
Produced by FastQC (version 0.11.2)