FastQCFastQC Report
Wed 8 Mar 2017
H5LWJBCXY_l02n01_mb_hho3_ntest_13.3510000007edc9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LWJBCXY_l02n01_mb_hho3_ntest_13.3510000007edc9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5551186
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG1045551.8834713879160236No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA701041.2628652687912096No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA432870.7797793120244936No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG295020.5314539991994504No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC279550.5035860805240538No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG234710.42281054895296244No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG229040.41259651541130127No Hit
GGCGGATCTTGAAGTTCACCTTGATGCCGTTCTTCTGCTTGTCGGCCATGA226240.407552548230234No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT223270.40220234018460194No Hit
ATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGT213920.3853590926335381No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGG208020.37473073321628925No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAG204430.36826364672342093No Hit
CTTTGCTCAGGGCGGACTGGGTGCTCAGGTAGTGGTTGTCGGGCAGCAGCA193500.34857416054875484No Hit
CGGATCTTGAAGTTCACCTTGATGCCGTTCTTCTGCTTGTCGGCCATGATA191520.34500735518500014No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAG181200.3264167332890665No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA175000.3152479488167033No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC173850.31317631943876495No Hit
CTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTG172950.31155504427342195No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTTG161310.29058655213498524No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATC152830.275310537243753No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA143570.2586294172092234No Hit
ATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGT140710.25347736501713325No Hit
GCGGATCTTGAAGTTCACCTTGATGCCGTTCTTCTGCTTGTCGGCCATGAT135270.24367765735105973No Hit
GTTTACGTCGCCGTCCAGCTCGACCAGGATGGGCACCACCCCGGTGAACAG117810.21222491914340466No Hit
GTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA115970.20891031213870334No Hit
CGCGCTTCTCGTTGGGGTCTTTGCTCAGGGCGGACTGGGTGCTCAGGTAGT110800.19959698702223272No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG109510.197273159285241No Hit
AGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGAT107850.1942828073136083No Hit
GTCGGGGTAGCGGCTGAAGCACTGCACGCCGTAGGTCAGGGTGGTCACGAG105670.19035571857977737No Hit
CCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTTGTACAGCTCGTCC103630.1866808282049998No Hit
GGCTGAAGCACTGCACGCCGTAGGTCAGGGTGGTCACGAGGGTGGGCCAGG103460.186374587340435No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC102550.18473529800658814No Hit
ATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTTGTACAG100870.1817089176979478No Hit
CTGAAGCACTGCACGCCGTAGGTCAGGGTGGTCACGAGGGTGGGCCAGGGC98800.17797998481765878No Hit
CCAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCAGCTCGATGCGGT96590.17399885357831643No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT92200.16609063360514312No Hit
GGGGTAGCGGCTGAAGCACTGCACGCCGTAGGTCAGGGTGGTCACGAGGGT90090.16228964405083887No Hit
TGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTA89940.1620194315232817No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGAT85850.15465163660522274No Hit
GGGGTCTTTGCTCAGGGCGGACTGGGTGCTCAGGTAGTGGTTGTCGGGCAG85280.15362482900050548No Hit
GTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACT83560.1505263920178499No Hit
CAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCAGCTCGATGCGGTT82220.14811249343833913No Hit
GGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTTGT81900.14753604004621715No Hit
GAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGA81500.14681547330606468No Hit
GCGGACTTGAAGAAGTCGTGCTGCTTCATGTGGTCGGGGTAGCGGCTGAAG80280.14461774474859967No Hit
CTTGAAGTTCACCTTGATGCCGTTCTTCTGCTTGTCGGCCATGATATAGAC79940.14400526301947006No Hit
AGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTG76240.13734002067305978No Hit
GTTGTACTCCAGCTTGTGCCCCAGGATGTTGCCGTCCTCCTTGAAGTCGAT75930.13678158144944164No Hit
GCCGTTTACGTCGCCGTCCAGCTCGACCAGGATGGGCACCACCCCGGTGAA74710.13458385289197658No Hit
ATGAACTTCAGGGTCAGCTTGCCGTAGGTGGCATCGCCCTCGCCCTCGCCG72900.1313232883927867No Hit
GTCTTTGCTCAGGGCGGACTGGGTGCTCAGGTAGTGGTTGTCGGGCAGCAG70270.12658556207628424No Hit
GGCGGACTGGGTGCTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTC66900.12051478729049972No Hit
CTTGTAGTTGCCGTCGTCCTTGAAGAAGATGGTGCGCTCCTGGACGTAGCC65530.11804684620547753No Hit
CGGCGAGCTGCACGCTGCCGTCCTCGATGTTGTGGCGGATCTTGAAGTTCA63940.11518259341337148No Hit
GTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGA63780.11489436671731049No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63570.11451606917873046No Hit
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA62600.11276869483386072No Hit
CTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGAACTCCAGCAGGAC62050.1117779155661511No Hit
GTAGCGGCTGAAGCACTGCACGCCGTAGGTCAGGGTGGTCACGAGGGTGGG61930.11156174554410535No Hit
TACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCA60440.10887763443703741No Hit
ACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAA59850.10781479849531253No Hit
GGGTGTTCTGCTGGTAGTGGTCGGCGAGCTGCACGCTGCCGTCCTCGATGT58690.10572515494887039No Hit
GGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTTGTACAGCTC58160.10477040401816837No Hit
GGATCTTGAAGTTCACCTTGATGCCGTTCTTCTGCTTGTCGGCCATGATAT57370.10334728470636725No Hit
GCGGACTGGGTGCTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCG56950.10259068962920716No Hit
GTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC56860.10242856211267286No Hit
TCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT56320.10145579701346705No Hit
TGAACTTCAGGGTCAGCTTGCCGTAGGTGGCATCGCCCTCGCCCTCGCCGG56040.1009514002953603No Hit
GGCTGTTGTAGTTGTACTCCAGCTTGTGCCCCAGGATGTTGCCGTCCTCCT55690.1003209043977269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG62300.036.1508525
TACTCTA62150.036.0932964
CTCTAGT62750.035.5330476
TTTTTCC143800.034.6703871
TCTAGTT65050.034.2417957
GTACTCT66150.034.0468333
CTAGTTG70150.031.5920038
TTTTCCA172850.028.924822
TGTTACC77000.028.72310813
GTTACCT76600.028.66749214
GTTGTTA78000.028.4125511
ACCTCTA78800.027.95278417
TACCTCT79800.027.74347516
TTGTTAC80000.027.73036212
TTACCTC79850.027.69792415
CCTCTAA79550.027.66096318
AGTTGTT82400.027.11381310
CTAATGC83600.026.18636121
TCTAATG83550.026.14817620
CTCTAAT86300.025.41923119
Produced by FastQC (version 0.11.2)