FastQCFastQC Report
Wed 8 Mar 2017
H5LWJBCXY_l02n01_mb_hho3_ntest_03.3510000007ec94.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LWJBCXY_l02n01_mb_hho3_ntest_03.3510000007ec94.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6041648
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG1279822.1183293035277795No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA995921.6484244034078117No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA685151.1340448831179837No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC435000.7200022245585973No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG382070.6323936780163293No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG307160.5084043294147557No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG297750.4928291088789019No Hit
ATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGT285690.47286766789458773No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT282760.46801799773836544No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAG272140.4504400123939693No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC270710.44807310852932847No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGG244170.4041446969436153No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA243640.4032674528539233No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAG237150.3925253506990146No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA201710.3338658591165854No Hit
ATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGT200840.3324258546674682No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATC177860.2943898750804416No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG174430.28871261616035887No Hit
CTTGACCTCAGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCTGCTT173480.28714019750902403No Hit
GTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA148980.24658834807986166No Hit
TGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTA130440.2159013567159159No Hit
GTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACT126920.21007513181833828No Hit
AGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGAT126070.20866823091977554No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGAT123000.20358683590967233No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC122640.2029909719996928No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACAC116860.19342404588946593No Hit
AGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTG110090.18221849402679535No Hit
GTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGA109730.18162263011681581No Hit
CTCGTCCATGCCGCCGGTGGAGTGGCGGCCCTCGGCGCGTTCGTACTGTTC101970.1687784525017015No Hit
GAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGA98470.16298533115467834No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT97750.1617936033347193No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACATGAACT97650.1616280855819472No Hit
CGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACATGAACTGAGGGGACA95630.15828462697595094No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86430.14305699372091854No Hit
GTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC84010.13905146410383393No Hit
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA83490.13819077178941905No Hit
TGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTA78720.1302955749821903No Hit
TCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT76250.12620728648871962No Hit
TACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCA70570.11680587813126485No Hit
ACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAA64650.10700722716715705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT84500.036.735921
TACTCTA85050.036.6391334
ACTCTAG84650.036.5996325
CTCTAGT86400.035.8062366
TCTAGTT86800.035.7449157
GTACTCT87700.035.557683
TTTTTCC175700.035.2837751
TGTACTC89250.034.8659632
CTAGTTG90650.033.8793148
TAGTTGT95700.032.1855749
TGTTACC100200.030.62784413
GTTACCT99750.030.5630114
GTTGTTA101800.030.10225911
TTGTTAC103000.029.81708312
AGTTGTT104100.029.6749210
CCTCTAA102900.029.4743518
TTTTCCA212250.029.4385152
TTACCTC104850.029.11931415
ACCTCTA105000.028.9277217
TACCTCT105800.028.81531516
Produced by FastQC (version 0.11.2)