FastQCFastQC Report
Mon 6 Mar 2017
H5LWJBCXY_l01n01_mb_biochip-dam_22.3510000007ef5e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LWJBCXY_l01n01_mb_biochip-dam_22.3510000007ef5e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9699830
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATG230149923.72720965212792TruSeq Adapter, Index 22 (97% over 40bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAA125708212.959835378558182No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA111560011.501232495827246No Hit
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATG572270.5899794120103136TruSeq Adapter, Index 22 (97% over 40bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAG425970.43915202637571993No Hit
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTG423730.43684270755260657Illumina Single End PCR Primer 1 (96% over 30bp)
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAG355990.3670064320714899No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAAGATCGGAAG341560.3521298826886657No Hit
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATG307530.3170467936035992TruSeq Adapter, Index 22 (97% over 40bp)
ATAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAA305850.3153148044862642No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAG267410.27568524396819327No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGCCG221270.22811739999567004TruSeq Adapter, Index 22 (97% over 37bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATG219180.22596272305803297TruSeq Adapter, Index 22 (97% over 40bp)
TCATCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA195310.20135404434923088No Hit
TATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATG150250.1548996219521373TruSeq Adapter, Index 22 (97% over 40bp)
GATGGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATG109990.11339373989028674TruSeq Adapter, Index 22 (97% over 40bp)
GATCCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTCCT109800.11319786016868337No Hit
GATCCCGCCAATCAGCTTCCTTGCGCCTTACGGGTTTACTCACCCGTTGAC109730.11312569395546107No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGC108180.11152772780553886TruSeq Adapter, Index 22 (97% over 39bp)
CGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCAC107360.11068235216493486No Hit
GATCACGGCAGCGACGTCTCCACAAGCGTATCAAAAGCCCGGGCTTAGGCC105610.1088781968343775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCCC20850.044.9409371
CTAATAC1652850.044.8990631
GATCCGC37200.044.806771
TCCTCGG1537550.044.766231
GATCAAT59550.044.6707231
GATCATC63650.044.65971
GATCAGC44350.044.6426621
GATCAAC49200.044.6369361
GATCCCA31850.044.6246451
GATCTCA58100.044.6225171
GATCCAA52400.044.619111
GATCGAG41600.044.6158031
GATCATT76400.044.606731
GATCGCT18650.044.5658651
GATCCAT55600.044.5628241
GATCTGC39950.044.541531
GATCTTA121350.044.5292431
GATCATG50600.044.514791
GATCTAC29250.044.509921
GATCGCC20600.044.5022541
Produced by FastQC (version 0.11.2)