FastQCFastQC Report
Mon 6 Mar 2017
H5LWJBCXY_l01n01_mb_biochip-dam_21.3510000007ef27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LWJBCXY_l01n01_mb_biochip-dam_21.3510000007ef27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7992890
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG223231727.92878420696394TruSeq Adapter, Index 21 (97% over 40bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAA116609114.589103565794098No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA105105613.14988696203751No Hit
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG549490.6874734920660738TruSeq Adapter, Index 21 (97% over 40bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAG420050.5255295644003608No Hit
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAG331180.4143432475612701No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAAGATCGGAAG319320.3995050601221836No Hit
ATAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAA290700.3636982368079631No Hit
CATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG289280.361921657873435TruSeq Adapter, Index 21 (97% over 40bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAG269690.3374123752485021No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATGCCG256400.3207850977556303TruSeq Adapter, Index 21 (97% over 37bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG223690.2798612266651987TruSeq Adapter, Index 21 (97% over 40bp)
TCATCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA179840.22499996872220185No Hit
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTG175020.21896960924021225Illumina Single End PCR Primer 1 (96% over 30bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG124240.15543814565194816TruSeq Adapter, Index 21 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATGC103340.12928990640431684TruSeq Adapter, Index 21 (97% over 39bp)
CCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA96680.12095750097899508No Hit
GATCCCGCCAATCAGCTTCCTTGCGCCTTACGGGTTTACTCACCCGTTGAC85800.10734540322711811No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGTATCTCGTATG81350.10177795515764637TruSeq Adapter, Index 21 (97% over 44bp)
TACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGC80810.10110235471775542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCC11550.045.048461
CTAATAC1509500.044.9559441
TCCTCGG1426350.044.8542251
GATCACC27250.044.8004881
GATCATG27850.044.7249531
GATCGTG19150.044.6956021
GATCCAT30150.044.6749231
GATCCTT35800.044.670961
GATCCAA29750.044.6699031
GATCCCA17600.044.6645241
GATCATT40050.044.654781
GATCCCT16450.044.6376841
GATCCTA14650.044.5872151
GATCGCT9750.044.5864221
GATCAAT33650.044.5799031
GATCATC36650.044.5567931
GATCGTC13350.044.5422971
GATCAAC26200.044.532641
GATCAGT19300.044.464931
GATCTCA30150.044.45081
Produced by FastQC (version 0.11.2)