FastQCFastQC Report
Mon 6 Mar 2017
H5LWJBCXY_l01n01_mb_biochip-dam_20.3510000007ef0d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LWJBCXY_l01n01_mb_biochip-dam_20.3510000007ef0d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6948859
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG202812129.186388729430256TruSeq Adapter, Index 20 (97% over 44bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAA106737215.360392260081834No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA93807913.49975585919933No Hit
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG494120.7110807687995973TruSeq Adapter, Index 20 (97% over 41bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTG435540.626779158995743Illumina Single End PCR Primer 1 (96% over 30bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAG398290.5731732360665255No Hit
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAG305500.43964052227854966No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAAGATCGGAAG288780.4155790180805223No Hit
CATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG287960.4143989682334899TruSeq Adapter, Index 20 (97% over 41bp)
ATAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAA273070.3929709899135959No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAG232910.33517732911259246No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCG222350.31998058961910153TruSeq Adapter, Index 20 (97% over 41bp)
CTATTACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAA194420.2797869405610331No Hit
GACCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG174450.25104840953025526TruSeq Adapter, Index 20 (97% over 41bp)
TCATCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA155260.22343236493933752No Hit
TATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG141800.2040622784258538TruSeq Adapter, Index 20 (97% over 41bp)
GATCCCGCCAATCAGCTTCCTTGCGCCTTACGGGTTTACTCACCCGTTGAC111740.1608033779358597No Hit
CCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA110480.15899013060993178No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGC94480.1359647677410061TruSeq Adapter, Index 20 (97% over 43bp)
GATGGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG93590.13468398193142211TruSeq Adapter, Index 20 (97% over 41bp)
GATCGTGGTCTGCAGCACGCGCCTAACGGCGTGCCTCGGCATCAGCGTGCT90950.1308847970580494No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCCTTATCTCGTATG87500.12591995318943727TruSeq Adapter, Index 20 (97% over 41bp)
TCCACGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA83300.1198757954363443No Hit
GTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAA78920.1135726023509759No Hit
GATCCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTCCT76640.11029148814215399No Hit
CGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCAC74930.10783065248553755No Hit
TACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGC74310.10693841967436668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCT6400.045.0454831
CTAATAC1359150.044.9659421
TCCTCGG1289800.044.8848341
GATCCAC11450.044.848781
GATCGTT8750.044.7880861
GATCAAT21850.044.7362481
GATCTAA12050.044.6716651
GATCTAC11000.044.6359831
GATCCAA19150.044.575041
GATCCAT25000.044.5049441
GATCTGG16350.044.4944731
GATCGCC8100.044.489371
GATCCAG16000.044.482421
GATCTTG30500.044.4547271
GATCATC22650.044.4488561
GATCAGT11200.044.44221
TCCGCGG67150.044.441751
GATCGAG20400.044.3830531
GATCCTG13350.044.3706471
GATCAAC17750.044.2841571
Produced by FastQC (version 0.11.2)