FastQCFastQC Report
Mon 6 Mar 2017
H5LWJBCXY_l01n01_mb_biochip-dam_19.3510000007eee2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LWJBCXY_l01n01_mb_biochip-dam_19.3510000007eee2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10696069
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG324513130.339473314915978TruSeq Adapter, Index 19 (97% over 40bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAA187870317.564424836825566No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA165181515.443196935247894No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAG907480.8484238461812467No Hit
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG848730.7934971249718004TruSeq Adapter, Index 19 (97% over 40bp)
TCCCCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA547590.5119544385886067No Hit
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAG535750.5008849512844392No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAAGATCGGAAG530750.49621033671342246No Hit
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTG525780.4915637698298319Illumina Single End PCR Primer 1 (96% over 30bp)
ATAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAA476060.4450794025356418No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAG452700.42323960325985177No Hit
GACCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG414090.38714222954246086TruSeq Adapter, Index 19 (97% over 40bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG412270.3854406698386108TruSeq Adapter, Index 19 (97% over 40bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCG393060.36748080065676464TruSeq Adapter, Index 19 (97% over 37bp)
CTCATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAA382480.35758931622449336No Hit
CTATTACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAA323510.3024569119739224No Hit
TCATCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA272070.2543644772673026No Hit
TATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG204350.19105149751745243TruSeq Adapter, Index 19 (97% over 40bp)
CCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA186270.17414809122865607No Hit
GATGGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG155040.14495044861808576TruSeq Adapter, Index 19 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGC151430.14157537689781172TruSeq Adapter, Index 19 (97% over 39bp)
TCCACGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA144330.134937424206968No Hit
GAACGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG142680.1333948013985325TruSeq Adapter, Index 19 (97% over 40bp)
GTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAA141290.13209525854778983No Hit
TACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGC133000.12434474758904415No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACTATCTCGTATG118430.1107229207291015TruSeq Adapter, Index 19 (97% over 44bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAAGATCGGAAGAGC115210.10771246894536676No Hit
CGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCAC110320.10314069589491243No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCT5250.045.044891
CTAATAC2417150.044.986191
TCCTCGG2292000.044.9132161
GATCCAT18100.044.7960241
GATCATG13450.044.709981
CGATCGC11900.044.62188340
TCCCCGG85800.044.3886381
GATCAAC13350.044.3700641
GATCAGG12450.044.3212781
TCCGCGG117450.044.3161931
GATCTTT15100.044.2991141
GATCATT26750.044.2871251
GATCATA14200.044.2518421
CCCCGGC91600.044.2377472
GATCCAC11000.044.225891
GATCTAT10800.044.2107241
GATCTAC7850.044.1841621
GATCAAT16800.044.1064571
ATGATCT9350.044.0374739
GATCCTT24400.044.0295331
Produced by FastQC (version 0.11.2)