FastQCFastQC Report
Mon 6 Mar 2017
H5LWJBCXY_l01n01_mb_biochip-dam_18.3510000007eec8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LWJBCXY_l01n01_mb_biochip-dam_18.3510000007eec8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4805129
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG391510.814775212070269TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC44750.042.68589833
ACGTCCG45000.042.5487532
TCCGCAC44550.042.47438435
CACGTCC45900.042.15562431
TCACGTC46950.041.93167530
GTCACGT47650.041.88229429
CGTCTGA49400.041.30951716
CATCTCG47000.041.21773541
ATCTCGT47850.041.1438542
ACGTCTG49550.041.13905315
ACACGTC50000.040.948813
GCACACG50100.040.8670611
TCTCGTA48300.040.80710643
CACACGT50300.040.6598412
CACATCT47000.040.64327239
TCTGAAC50350.040.4407218
GAGCACA51400.039.789699
AGCACAC51550.039.761210
AGAGCAC52000.039.3738448
TCGGAAG52750.036.6387333
Produced by FastQC (version 0.11.2)