Basic Statistics
Measure | Value |
---|---|
Filename | H5LWJBCXY_l01n01_mb_biochip-dam_17.3510000007eeaf.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 8951172 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCC | 337898 | 3.7749023256395926 | TruSeq Adapter, Index 3 (97% over 37bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTATGCC | 41070 | 0.0 | 43.04931 | 45 |
CGTATGC | 41195 | 0.0 | 42.962376 | 44 |
CGTCTGA | 46115 | 0.0 | 42.85187 | 16 |
ACGTAGA | 40780 | 0.0 | 42.824825 | 32 |
CGTAGAG | 40700 | 0.0 | 42.64365 | 33 |
GTCACGT | 42780 | 0.0 | 42.626667 | 29 |
TCTCGTA | 41100 | 0.0 | 42.558273 | 41 |
AGTCACG | 43335 | 0.0 | 42.490902 | 28 |
TCACGTA | 42225 | 0.0 | 42.371696 | 30 |
CACGTAG | 41760 | 0.0 | 42.369385 | 31 |
ATCTCGT | 41110 | 0.0 | 42.279736 | 40 |
CTCGTAT | 41850 | 0.0 | 42.278976 | 42 |
ACGTCTG | 46855 | 0.0 | 42.242325 | 15 |
TCGTATG | 42090 | 0.0 | 42.1985 | 43 |
ACACGTC | 47000 | 0.0 | 42.174232 | 13 |
CACACGT | 47410 | 0.0 | 41.785786 | 12 |
TCTGAAC | 47345 | 0.0 | 41.75286 | 18 |
GCACACG | 47675 | 0.0 | 41.591274 | 11 |
GTCTGAA | 47795 | 0.0 | 41.364452 | 17 |
CACGTCT | 48135 | 0.0 | 41.179783 | 14 |