FastQCFastQC Report
Mon 6 Mar 2017
H5LWJBCXY_l01n01_mb_biochip-dam_17.3510000007eeaf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LWJBCXY_l01n01_mb_biochip-dam_17.3510000007eeaf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8951172
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCC3378983.7749023256395926TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC410700.043.0493145
CGTATGC411950.042.96237644
CGTCTGA461150.042.8518716
ACGTAGA407800.042.82482532
CGTAGAG407000.042.6436533
GTCACGT427800.042.62666729
TCTCGTA411000.042.55827341
AGTCACG433350.042.49090228
TCACGTA422250.042.37169630
CACGTAG417600.042.36938531
ATCTCGT411100.042.27973640
CTCGTAT418500.042.27897642
ACGTCTG468550.042.24232515
TCGTATG420900.042.198543
ACACGTC470000.042.17423213
CACACGT474100.041.78578612
TCTGAAC473450.041.7528618
GCACACG476750.041.59127411
GTCTGAA477950.041.36445217
CACGTCT481350.041.17978314