FastQCFastQC Report
Mon 6 Mar 2017
H5LWJBCXY_l01n01_mb_biochip-dam_16.3510000007ee85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LWJBCXY_l01n01_mb_biochip-dam_16.3510000007ee85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5262308
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCC1277452.427547000289607TruSeq Adapter, Index 16 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC153100.042.3254345
CCCGTCC151550.042.11831333
ACGTCTG167750.041.77948815
GCACACG169000.041.7500411
CGTCTGA167950.041.7163416
CGTATGC155700.041.690944
CCGTCCA152150.041.62689634
CACCCGT156650.041.5801231
CACACGT170000.041.4647512
TCACCCG157600.041.45796630
CCAGTCA165750.041.40128726
CACGTCT169800.041.3413314
AGTCACC161850.041.2729128
CAGTCAC165350.041.22930527
TCCAGTC168450.041.2185325
CGTCCAT154150.041.16057235
ACACGTC171300.041.1106713
GTCACCC160550.041.07457729
ACCCGTC156850.041.0680832
CTCGTAT158550.040.78542
Produced by FastQC (version 0.11.2)