FastQCFastQC Report
Mon 6 Mar 2017
H5LWJBCXY_l01n01_mb_biochip-dam_15.3510000007ee78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LWJBCXY_l01n01_mb_biochip-dam_15.3510000007ee78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6180294
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG484340.7836844007744614TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA75100.039.87529816
ACGTCTG76650.039.15700515
CTCGTAT65600.039.06733744
TCGTATG65850.039.02152345
CACACGT77000.039.0082412
ACACGTC78550.038.23850313
GCACACG79150.038.03391311
CACGTCT79150.038.00548614
CAGTCAC73100.037.70374727
AGTCACA67900.037.21138428
TCTCGTA69150.037.19186443
CCAGTCA74550.037.09112526
GTCTGAA80900.037.016517
CTCCAGT80300.036.9288424
ACTCCAG80850.036.92807823
TCTGAAC81400.036.76148218
GTCACAT67100.036.68264429
TCCAGTC81100.036.56456425
ATGTCAG65050.036.48975834
GAACTCC82400.036.23343721
Produced by FastQC (version 0.11.2)