FastQCFastQC Report
Mon 6 Mar 2017
H5LWJBCXY_l01n01_mb_biochip-dam_14.3510000007ee5f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LWJBCXY_l01n01_mb_biochip-dam_14.3510000007ee5f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7935391
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG179420.2261010201009629TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG26200.039.76011311
ACGTCTG26150.039.750115
CACACGT26700.039.0155412
ACACGTC26700.038.93127413
CGTCTGA26800.038.8699616
GTTCCGT22350.038.75732835
GAGCACA27400.038.2651379
AGTTCCG22600.038.02969734
TCACAGT23400.037.97949230
CGTATCT23150.037.9046539
AGCACAC27700.037.68826710
AGAGCAC27800.037.6336258
TTCCGTA23400.037.40282436
TCGGAAG27200.037.3891563
CACAGTT23500.037.3391731
TCTCGTA25350.037.27779443
TCTGAAC27950.037.2706618
TCCGTAT23750.037.04109637
ATCTCGT25450.036.86609642
TCGTATG43000.022.34297845
Produced by FastQC (version 0.11.2)