FastQCFastQC Report
Mon 6 Mar 2017
H5LWJBCXY_l01n01_mb_biochip-dam_13.3510000007ee35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LWJBCXY_l01n01_mb_biochip-dam_13.3510000007ee35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6529523
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG1850842.8345715299570884TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG233650.041.08139445
TCTCGTA236750.040.61919843
CGTCTGA252000.040.3824716
ATCTCGT238500.040.33027642
CTCGTAT240300.040.0378544
AATCTCG238300.039.9770141
AGTCAAC234100.039.7892534
ACGTCTG256400.039.70702715
CACAGTC238300.039.60725831
TCTGAAC257150.039.52997618
ACAGTCA237850.039.4173332
ACACGTC260200.039.20496413
GCACACG260850.039.14177311
CACACGT260800.039.13202312
TCACAGT244350.038.93045430
AACAATC243650.038.80369638
CTGAACT262100.038.79219
GTCAACA241000.038.6512435
GTCTGAA263450.038.63591817
CAGTCAA242700.038.61109533
Produced by FastQC (version 0.11.2)