FastQCFastQC Report
Mon 6 Mar 2017
H5LWJBCXY_l01n01_mb_biochip-dam_12.3510000007ee1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LWJBCXY_l01n01_mb_biochip-dam_12.3510000007ee1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5052575
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC453300.8971662963934232TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC51550.041.7697545
CGTATGC53100.040.63522344
CGTCTGA57500.040.5770916
AGTCACC55100.040.50728
CACACGT58100.040.39040412
GCACACG58050.040.38643311
AATCTCG53550.040.25094239
TCTGAAC57800.040.24970218
ACACGTC58200.040.24368713
ACGTCTG58000.040.18849615
ATCTCGT53850.040.11026440
TCTCGTA54150.039.84649741
TGTAATC53550.039.66272436
AGCACAC59400.039.43068310
AGAGCAC59500.039.4022258
GAGCACA59450.039.3596769
CTTGTAA54050.039.2541934
TCGGAAG58300.038.2836273
GTCACGT1600.035.15523529
CTGAACT69300.033.7327919
Produced by FastQC (version 0.11.2)