FastQCFastQC Report
Mon 6 Mar 2017
H5LWJBCXY_l01n01_mb_biochip-dam_11.3510000007edf0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LWJBCXY_l01n01_mb_biochip-dam_11.3510000007edf0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6059936
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC1598732.638196178969547TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC193700.042.08428645
CGTATGC198500.041.08930244
TCTCGTA199100.040.76307341
CGTCTGA214750.040.56664316
CTCGTAT201800.040.41770642
CACGGCT202850.040.40648731
AGTCACG207950.040.3784528
CATCTCG200400.040.374839
ATCTCGT201350.040.35225740
ACGGCTA202400.040.31846232
TCGTATG203450.040.24441543
CGGCTAC202450.040.17514433
GCTACAT201500.040.00856835
GTCACGG207150.039.96959729
CTACATC201800.039.8602236
ACGTCTG220000.039.67016215
TCACGGC208050.039.59122530
TCTGAAC221150.039.3824918
ACACGTC222550.039.2661713
TACATCT206050.039.12542337
Produced by FastQC (version 0.11.2)