FastQCFastQC Report
Mon 6 Mar 2017
H5LWJBCXY_l01n01_mb_biochip-dam_10.3510000007edd6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LWJBCXY_l01n01_mb_biochip-dam_10.3510000007edd6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7991250
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC424720.5314813076802753TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC52150.041.46204845
CGTATGC54200.039.89383344
ATCTCGT54050.039.5882740
TCTCGTA54300.039.40641
GTCACTA54850.039.337829
CACTAGC54400.039.33233331
TAGCTTA54100.039.3011634
AGTCACT55850.039.03630428
ACTAGCT54700.039.0343532
AGCTTAT54500.038.88934735
CGTCTGA58400.038.7187516
ACGTCTG58600.038.62499615
TCTGAAC58650.038.51534318
GCTTATC55250.038.1985436
CACACGT59500.038.15419812
ACACGTC59600.038.1279313
TTATCTC55850.038.0704138
GCACACG60100.037.81072611
GAGCACA61400.037.0834589
AGCACAC61800.036.8070310
Produced by FastQC (version 0.11.2)