FastQCFastQC Report
Mon 6 Mar 2017
H5LWJBCXY_l01n01_mb_biochip-dam_09.3510000007edbc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LWJBCXY_l01n01_mb_biochip-dam_09.3510000007edbc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6302301
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC2904174.608110593257923TruSeq Adapter, Index 9 (100% over 51bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTG105480.16736744246268148Illumina Single End PCR Primer 1 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC354000.042.59060745
CGTATGC365400.041.3480444
TCTCGTA364250.041.02213741
CGTCTGA392000.040.87737716
TCTGAAC392550.040.82010318
ATCTCGT366400.040.6893140
TCACGAT370250.040.62921530
CACGATC369350.040.47846231
AGTCACG383900.040.4680928
CTCGTAT373550.040.3676242
TCGTATG376150.040.28598843
CTGAACT398650.040.1785619
GTCACGA378500.040.04085529
GTCTGAA401400.039.92571317
ACGTCTG403000.039.8230315
ACTCCAG402700.039.6962623
CAGATCT370750.039.67690737
AACTCCA403200.039.62471422
CAGTCAC395600.039.61816427
CTCCAGT403900.039.4780524
Produced by FastQC (version 0.11.2)