FastQCFastQC Report
Mon 6 Mar 2017
H5LWJBCXY_l01n01_mb_biochip-dam_08.3510000007ed93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LWJBCXY_l01n01_mb_biochip-dam_08.3510000007ed93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12048496
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC730940.6066649314570051TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC90600.041.2250345
CGTATGC92750.040.31792444
GTCACAC94300.039.7733729
AATCTCG92750.039.6385239
TCTCGTA93800.039.57859841
CGTCTGA98750.039.5303716
TCACACT94850.039.3766930
CACACTT94850.039.32924731
ATCTCGT94500.039.21399340
ACGTCTG100450.038.8613615
TCTGAAC100500.038.77486418
CTGAACT101050.038.5415519
ACACGTC102850.038.10766213
GCACACG103100.038.0152611
CACACGT103800.037.80224612
AGCACAC107900.036.38667710
GAGCACA108750.035.9160779
AGAGCAC109800.035.61368
TCGGAAG116100.032.0923843
ACTCCAG129800.030.3861523
Produced by FastQC (version 0.11.2)