FastQCFastQC Report
Mon 6 Mar 2017
H5LWJBCXY_l01n01_mb_biochip-dam_07.3510000007ed86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LWJBCXY_l01n01_mb_biochip-dam_07.3510000007ed86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7523048
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC1497461.9904964051804535TruSeq Adapter, Index 7 (100% over 51bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTG76270.10138178036349096Illumina Single End PCR Primer 1 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC189650.041.27549745
CGTATGC195550.040.11070344
TCTCGTA195300.039.97718441
CGTCTGA209750.039.549316
CTCGTAT199450.039.33740642
CATCTCG196700.039.31490339
AGTCACC202950.039.15608628
ATCTCGT200400.038.93734440
TCTGAAC214300.038.7200918
CTGAACT215100.038.61792419
CTCCAGT215400.038.2298924
ACGTCTG217600.038.15356415
TCGTATG206300.038.14056443
GTCTGAA219500.037.85405717
CACCAGA206250.037.72234331
CAGTCAC214000.037.6494127
ACTCCAG220550.037.56162323
ACCAGAT206400.037.32430332
ACACGTC223250.037.22828713
GTCACCA211650.037.11070629
Produced by FastQC (version 0.11.2)