FastQCFastQC Report
Mon 6 Mar 2017
H5LWJBCXY_l01n01_mb_biochip-dam_06.3510000007ed50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LWJBCXY_l01n01_mb_biochip-dam_06.3510000007ed50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7076376
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1781702.517814203202317TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC214300.042.4478645
CGTATGC223450.040.7398844
TCTCGTA224100.040.36067241
ATCTCGT224300.040.35477440
CGCCAAT224700.040.22257633
TCACGCC227900.040.19091430
CACGCCA227500.040.1626831
CCAATAT223500.040.1468835
GCCAATA224550.040.09914834
CTGAACT234800.039.9489119
CAATATC224100.039.92895536
TCTGAAC234750.039.92866518
CGTCTGA235800.039.7508716
ACGTCTG237800.039.4543915
CACACGT240050.039.16893812
ACACGTC240000.039.1489713
GCACACG241200.039.02882811
TATCTCG233000.038.78037339
AGCACAC243350.038.7209910
GAGCACA243700.038.5915229
Produced by FastQC (version 0.11.2)