FastQCFastQC Report
Mon 6 Mar 2017
H5LWJBCXY_l01n01_mb_biochip-dam_05.3510000007ed36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LWJBCXY_l01n01_mb_biochip-dam_05.3510000007ed36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7411040
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC78746210.625526241930958TruSeq Adapter, Index 5 (100% over 51bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTG271320.3661024633519722Illumina Single End PCR Primer 1 (96% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC167360.22582525529480343TruSeq Adapter, Index 5 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCG127990.1727018070338306TruSeq Adapter, Index 5 (100% over 51bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC99370.13408374533128953TruSeq Adapter, Index 5 (98% over 51bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC95490.12884831278740908TruSeq Adapter, Index 5 (98% over 51bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTC93690.12641950387529954TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC940950.042.9070245
CGTATGC970950.041.62301344
TCTCGTA964650.041.5231341
ATCTCGT965250.041.3481340
CTCGTAT976450.041.3030242
GTCACAC984150.041.1185729
AGTGATC959000.041.0583736
CAGTGAT961950.041.04472735
TCTGAAC1032300.040.98785818
TCGTATG989350.040.97854243
ACACAGT973750.040.96853632
ACAGTGA965000.040.8975834
CTGAACT1034350.040.89356619
CTCCAGT1030600.040.88736724
CGTCTGA1036000.040.87838716
GTGATCT965500.040.82856437
CACAGTG972950.040.8241633
GTCTGAA1040400.040.69469517
AGTCACA1000650.040.6878828
CAGTCAC1026700.040.65041727
Produced by FastQC (version 0.11.2)